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KMID : 0364819870250040255
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Korean Journal of Microbiology
1987 Volume.25 No. 4 p.255 ~ p.261
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Isolation and characterization of plasmids isolated from Streptomyces spp. And construction of recombinant plasmids
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Yu, Ju-Hyun/유주현
Yum, Do-Young/Kong, In-Soo/염도영/공인수
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Abstract
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1
Five independent Actinomycetes harboring plasmids were isolated from soil. Molecular weight of these plasmids was 55 kb, 6.2 kb, 4.4 kb, 55 kb and 7.0 kb, respectively. Among them, small and apprent high copy number plasmids, pJY501 of 4.4 kb and pJY711 of 7.0 kb, were selected. The plasmids purified by CsCl-EtBr density gradient centrifugation preserved the conformation of supercoiled covalently dosed circular molecule, and an apparent copy number was estimated about 150 and about 35 per chromosome. The isolates carrying plasmids were assigned to the genus Streptomyces. For the purpose of introducing selection markers into the isolated plasmids, the tsr fragment of pIJ702 was inserted into the Bcll site of pJY501 and pJY711. And the recombinant plasmids constructed designated as pJY502 and pJY712 respectively.
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KEYWORD
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